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Merck

LSKMAGA

PureProteome Protein A Magnetic Bead System

The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability

Sinónimos:

Protein A Magnetic Beads

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Código UNSPSC:
41105507
eCl@ss:
32160405
NACRES:
NA.56

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Nivel de calidad

envase

pkg of 2 × 1 mL

fabricante / nombre comercial

PureProteome

técnicas

depletion: suitable (serum)
immunoprecipitation (IP): suitable
protein purification: suitable

tamaño de partícula

10 μm

capacidad

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

Condiciones de envío

wet ice

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Este artículo
LSKMAGGLSKMAGHLSKMAGAG
packaging

pkg of 2 × 1 mL

packaging

pkg of 2 × 1 mL

packaging

pkg of 10 mL

packaging

pkg of 2 × 1 mL

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

manufacturer/tradename

PureProteome

technique(s)

depletion: suitable (serum)

technique(s)

depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable

technique(s)

protein purification: suitable (histidine tagged recombinant protein)

technique(s)

immunoprecipitation (IP): suitable (crosslinking), protein purification: suitable (antibody)

particle size

10 μm

particle size

10 μm

particle size

10 μm

particle size

10 μm

capacity

1.5-2.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

2.5-3.5 μg/μL, suspension binding capacity (rabbit IgG)

capacity

1-5.5 mg/mL, bead suspension binding capacity (protein)

capacity

2-3 mg/mL, suspension binding capacity (rabbit IgG)

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

Descripción general

The PureProteome Protein A Magnetic Bead System is a powerful system that helps researchers in protein purification by maximizing recovery and reducing variability.

Aplicación

PureProteome Protein A Magnetic Bead System may be used:
  • in serum depletion, in immunoprecipitation, for protein purification, and in cell lysates to analyze the bound proteins by western blot[1]
  • in magnetic bead-binding assays[1]
  • for immunoprecipitating cell lysate and antibodies for ATPase activity[1]

Información legal

PureProteome is a trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Kris Wilson et al.
Journal of biomolecular screening, 20(2), 292-298 (2014-10-10)
Human kynurenine 3-monooxygenase (KMO) is emerging as an important drug target enzyme in a number of inflammatory and neurodegenerative disease states. Recombinant protein production of KMO, and therefore discovery of KMO ligands, is challenging due to a large membrane targeting
Konstantin Chegaev et al.
ACS medicinal chemistry letters, 8(3), 361-365 (2017-03-25)
Nitric oxide (NO) release from a suitable NO photodonor (NOP) can be fine-tuned by visible light stimuli at doses that are not toxic to cells but that inhibit several efflux pumps; these are mainly responsible for the multidrug resistance of
Fei-Yun Chen et al.
The Journal of cell biology, 218(9), 3002-3018 (2019-08-08)
The BH3-only pro-apoptotic protein BIK is regulated by the ubiquitin-proteasome system. However, the mechanism of this regulation and its physiological functions remain elusive. Here, we identify Cul5-ASB11 as the E3 ligase targeting BIK for ubiquitination and degradation. ER stress leads
Holger Laux et al.
Biotechnology and bioengineering, 115(10), 2530-2540 (2018-05-20)
An increasing number of nonantibody format proteins are entering clinical development. However, one of the major hurdles for the production of nonantibody glycoproteins is host cell-related proteolytic degradation, which can drastically impact developability and timelines of pipeline projects. Chinese hamster
Thibaut Hacquard et al.
Cell reports, 39(2), 110671-110671 (2022-04-14)
RNA silencing is a conserved mechanism in eukaryotes involved in development and defense against viruses. In plants, ARGONAUTE1 (AGO1) protein plays a central role in both microRNA- and small interfering RNA-directed silencing, and its expression is regulated at multiple levels.

Artículos

The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.

PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.

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Preguntas

  1. What is the concentration of the beads?

    1 respuesta
    1. The beads are not characterized in terms of beads/ml but only in terms of a percentage slurry (10%, weight [g] by volume [mL]).

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