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Materiales
self-standing
Características
binder
fabricante / nombre comercial
PureProteome
técnicas
RNA purification: suitable (with magnetic beads)
protein purification: suitable
Condiciones de envío
ambient
Categorías relacionadas
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Este artículo | LSKMAGL10 | LSKMAGS08 | LSKMAGG |
|---|---|---|---|
| feature binder | feature - | feature binder | feature - |
| manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome | manufacturer/tradename PureProteome |
| material self-standing | material - | material self-standing | material - |
| technique(s) RNA purification: suitable (with magnetic beads) | technique(s) depletion: suitable (serum), protein purification: suitable | technique(s) RNA purification: suitable (with magnetic beads), protein purification: suitable | technique(s) depletion: suitable (serum), immunoprecipitation (IP): suitable, protein purification: suitable |
| shipped in ambient | shipped in wet ice | shipped in ambient | shipped in wet ice |
Descripción general
Aplicación
- purification with magnetic beads
- protein purification
- in the purification of the heat shock protein 90 (Hsp90)/ Cdc37 (cell division cycle 37)/ Cyclin-dependent kinase 4 (Cdk4) complex to incubate and wash the beads with 10 bed volumes of lysis buffer[1]
Otras notas
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
Read an automated protocol for protein purification using PureProteome™ nickel magnetic beads on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.
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