MAB2160

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3

ascites fluid, clone 1C3, Chemicon^®

• Sinónimos: FMRP
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 1C3, monoclonal
• reactividad de especies: mouse, human, rat
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG1κ
• idoneidad: not suitable for flow cytometry; not suitable for immunoprecipitation
• Nº de acceso NCBI: NM_002024.3
• Nº de acceso UniProt: Q06787
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FRAXA(2477), FRAXA(108684022)

Descripción

Descripción general

Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.

~71 kDa

Inmunógeno

Fusion protein with a full length FMRP (human).

Aplicación

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.

Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.

Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

RNA Binding Protein (RBP)

Acciones bioquímicas o fisiológicas

Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.

Forma física

Ascites mouse monoclonal IgG1k fluid containing no preservatives

Unpurified

Nota de preparación

Stable for 1 year at -20ºC from date of receipt.

Nota de análisis

Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate

Evaluated by Western Blot on Mouse E17 spinal cord lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable