MAB4037
Anti-PTEN Antibody, CT, clone A2b1
clone A2b1, Chemicon®, from mouse
Sinónimos:
MMAC1, TEP1
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Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Permítanos ayudarleorigen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
A2b1, monoclonal
reactividad de especies
human, rat, mouse
fabricante / nombre comercial
Chemicon®
técnicas
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG1
idoneidad
not suitable for immunohistochemistry (Paraffin)
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... PTEN(5728), TEP1(7011)
1 of 4
Este artículo | P3487 | AMAB91736 | AMAB91735 |
|---|---|---|---|
| species reactivity human, rat, mouse | species reactivity mouse, human, rat | species reactivity human | species reactivity human |
| clone A2b1, monoclonal | clone PTN-18, monoclonal | clone CL11723, monoclonal | clone CL11722, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Gene Information human ... PTEN(5728), TEP1(7011) | Gene Information human ... PTEN(5728) | Gene Information human ... PTEN(5728) | Gene Information human ... PTEN(5728) |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Descripción general
55 kDa
Inmunógeno
Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus
Aplicación
Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Acciones bioquímicas o fisiológicas
Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
Forma física
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Nota de preparación
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análisis
Control
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-409
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Carsten B Schmidt-Weber et al.
European journal of immunology, 32(4), 1196-1204 (2002-04-05)
The phosphatidylinositol phosphatase gene PTEN is a dual specific phosphatase acting on phospho amino acids but also on three phosphorylated inositol phospholipids. Present results demonstrate that PTEN is inducible by costimulatory signals in human CD4(+) T cells. PTEN expression was
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FEBS letters, 586(23), 4108-4113 (2012-11-06)
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