MABE664

Anti-CBX1 (HP1 beta) Antibody, clone 5E9.1

clone 5E9.1, from mouse

• Sinónimos: Chromobox protein homolog 1, Heterochromatin protein 1 homolog beta, HP1 beta, Heterochromatin protein p25, M31, Modifier 1 protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 5E9.1, monoclonal
• reactividad de especies: human
• envase: antibody small pack of 25 μg
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001120700
• Nº de acceso UniProt: P83916
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CBX1(10951)

Descripción

Descripción general

Chromobox protein homolog 1 (UniProt: P83916; also known as HP1Hsbeta, heterochromatin protein 1 homolog beta, heterochromatin protein p25, M31, Modifier 1 protein, p25 beta) is encoded by the CBX1 (also known as CBX) gene (Gene ID:10951) in human. Chromobox protein homolog 1 is a homodimeric nuclear protein that is a component of heterochromatin. It is expressed in all adult and embryonic tissues. It is essential for formation of and gene repression in heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Also interacts with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. The human HP1 homolog CBX1 preferentially recognize H3K9me3 (Peptide binding specificity Kd = 5 µM). However, upon substitution of Threonine 6 to Alanine reduces its affinity significantly (Kd = 45 mM). (Ref.: Kaustov, L., et al. (2011). J. Biol. Chem. 286(1): 521-529).

~23 kDa observed; 21.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Inmunógeno

GST/His-tagged recombinant protein fragment corresponding to 60 amino acids from the internal region of human Chromobox protein homolog 1 (CBX1).

Aplicación

Anti-CBX1 (HP1 beta) antibody, clone 5E9.1, Cat. No. MABE664, is a highly specific mouse monoclonal antibody that targets CBX1 and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Acciones bioquímicas o fisiológicas

Clone 5E9.1 specifically detects human Chromobox protein homolog 1 (CBX1). It targets an epitope with in 60 amino acids from the internal region.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Nota de preparación

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CBX1 (HP1 beta) in 10 µg of HeLa cell lysate.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash