MABF1986M

Anti-NKR-P1G Antibody, clone ADA3

clone ADA3, from hamster(Armenian)

• Sinónimos: Killer cell lectin-like receptor subfamily B member 1, Immunoreceptor NKR-P1E, Killer cell lectin-like receptor subfamily B member 1G, Natural killer cell surface protein NKR-P1G, Natural killer lectin-like receptor 1E
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: hamster (Armenian)
• Nivel de calidad: 100
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: ADA3, monoclonal
• reactividad de especies: rat
• envase: antibody small pack of 25 μL
• técnicas: flow cytometry: suitable; immunoprecipitation (IP): suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_001078874.1
• Nº de acceso UniProt: Q0ZUP0
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: rat ... Klrb1(689817)

Descripción

Descripción general

23.96 kDa calculated.

Killer cell lectin-like receptor subfamily B member 1 (UniProt: Q0ZUP0; also known as Immunoreceptor NKR-P1E, Killer cell lectin-like receptor subfamily B member 1G, Natural killer cell surface protein NKR-P1G, Natural killer lectin-like receptor 1E) is encoded by the Klrb1 (also known as Klrb1d, Klrb1g, Klrb6, Nkrp1e, Nkrp1g) gene (Gene ID: 689817) in rat. NKR-P1G is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-42), a helical domain (aa 43-63), and an extracellular domain (aa 64-214). NKR-P1G is expressed by subsets of lymphocytes with highest prevalence in gut and liver mucosa. The number of NKR-P1G+ cells are shown to increase with age and its expression level is modulated by cytokines, with an increase induced by inflammatory cytokines. It is shown to be mainly expressed by Ly49s3+ cells. It is reported to inhibit cytotoxicity and calcium responses in IL-2-activated NK cells. NKR-P1G is a potent inhibitory receptor for a panel of Clr ligands. The intracellular domain of NKR-P1G contains an immunoreceptor tyrosine-based inhibitory motif that is phosphorylated by Src-kinases upon activation. This allows recruitment of phosphotyrosine phosphatases, which in turn negatively regulates the signaling cascades that is essential for cytotoxicity and cytokine production. (Ref.: Kveberg, L et al (2015). Eur J Immunol. 45(2); 501-512).

Inmunógeno

Chinese hamster ovary (CHO) cells stably transfected with FLAG-tagged extracellular portion of NKR-P1G.

Epitope: extracellular domain

Aplicación

Anti-NKR-P1G, clone ADA3, Cat. No. MABF1986 is an Armenian hamster monoclonal antibody that detects NKG-P1G and has beent tested for use in Flow Cytometry and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot detected NKR-PIG in Immunoprecipitation applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).

Flow Cytometry Analysis: A representative lot detected NKR-PIG in Flow Cytometry applications (Kveberg, L., et. al. (2015). Eur J Immunol. 45(2):501-12).

Research Category
Inflammation & Immunology

Acciones bioquímicas o fisiológicas

Clone ADA3 is an Armenian hamster monoclonal antibody that detects Killer cell lectin-like receptor subfamily B member 1 (NKR-P1G) in rat. It targets an epitope within the extracellular domain.

Forma física

Format: Purified

Protein G purified

Purified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Nota de preparación

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Evaluated by Flow Cytometry in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.

Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1G in Ltk (mouse fibroblast) cells stably transfected with FLAG-tagged rat NKR-P1G.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable