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Merck

MR-106

EmbryoMax® KSOM Mouse Embryo Media

(1X), Liquid, with 1/2 Amino Acids

Sinónimos:

K+ Simplex Optimised Medium (KSOM), KSOM Mouse Embryo Media

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Código UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.71

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Nivel de calidad

300

Formulario

liquid

fabricante / nombre comercial

Specialty Media
EmbryoMax®

técnicas

cell culture | embryo: suitable
cell culture | stem cell: suitable

entrada

sample type: mouse embryo(s)

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Este artículo
MR-121MR-101MR-015
EmbryoMax® KSOM Mouse Embryo Media (1X), Liquid, with 1/2 Amino Acids

Sigma-Aldrich

MR-106

EmbryoMax® KSOM Mouse Embryo Media

EmbryoMax® KSOM Mouse Embryo Media (1X), Liquid, with 1/2 Amino Acids & Phenol Red

Sigma-Aldrich

MR-121

EmbryoMax® KSOM Mouse Embryo Media

EmbryoMax® Advanced KSOM Embryo Medium EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.

Sigma-Aldrich

MR-101

EmbryoMax® Advanced KSOM Embryo Medium

M2 medium EmbryoMax M2 Medium (1X), Liquid, with Phenol Red

Sigma-Aldrich

MR-015

M2 medium

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | embryo: suitable, cell culture | stem cell: suitable

form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

manufacturer/tradename

Specialty Media

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

manufacturer/tradename

Specialty Media, EmbryoMax®

input

sample type: mouse embryo(s)

input

sample type: mouse embryo(s)

input

sample type: mouse embryo(s)

input

sample type: mouse embryo(s)

Aplicación

EmbryoMax® KSOM Mouse Embryo Media has been used in the culturing of mouse embryos for embryonic development.[1][2]

Nota de preparación

Can be stored at -20°C upto the expiration date on the bottle.


Once thawed it should be used within 2 weeks.

Información legal

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Benjamin L Kidder
Methods in molecular biology (Clifton, N.J.), 1150, 191-199 (2014-04-20)
Epigenetic regulation of gene expression in the germline is important for reproductive success of mammals. Misregulation of genes whose expression is correlated with reproductive success may result in subfertility or infertility. To study epigenetic events that occur during oocyte maturation
Yilong Miao et al.
STAR protocols, 2(1), 100298-100298 (2021-02-04)
The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide

Artículos

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

Preguntas

1–5 de 5 Preguntas  

  1. Could you please send me the medium composition? We need to know the concentration of BSA, essential amino acids and non-essential amino acids, please

    1 respuesta

    1. This medium is listed as a 1/2 amino acid formulation. The complete list of components as well as the concentration of each are listed below:
      Essential and Non-essential amino acids are also designated.
      MR-106-D EmbryoMax KSOM Mouse Embryo Medium
      Component - mg/L – E = Essential, N = Non-Essential
      CaCl2-2H2O 250.00
      KCL 186.38
      KH2PO4 47.99
      MgSO4 7H2O 49.30
      NaCl 5551.80
      NaHCO3 2100.25
      EDTA 3.72
      D-Glucose 36.03
      Sodium Lactate 1121.00
      Lactate Na Salt 1.42 (ml)
      Sodium Pyruvate 22.00
      Bovine Serum Albumin 1000.00
      L-Arginine 63.20 E Conditionally essential
      L-Cystine 12.02 N Conditionally essential
      L-Glutamine 146.15 N Conditionally essential
      Glycine 3.75 N Conditionally essential
      L-Histidine·HCl·H2O 20.96 E
      L-Isoleucine 26.23 E
      L-Leucine 26.24 E
      L-Lysine·HCl 36.52 E
      L-Methionine 7.46 E
      L-Phenylalanine 16.52 E
      L-Serine 5.26 N Conditionally essential
      L-Threonine 23.82 E
      L-Tryptophan 5.11 E
      L-Tyrosine 18.12 N Conditionally essential
      L-Valine 23.42 E
      L-Alanine 4.45 N
      L-Asparagine·H2O 7.50 N
      L-Aspartic Acid 6.66 N
      L-Glutamic Acid 7.36 N
      L-Proline 5.76 N
      Pen G Na Salt 100,000.00 (units)
      Strep Sulfate 50.00

      ¿Le ha resultado útil?

  2. Hello, I have been working with the KSOM MR-106-D for some time and I would need to know the exact composition of all the components of this medium, could you please provide it to me. Thank you

    1 respuesta

    1. Please see the image below for the full formulation of this product:

      ¿Le ha resultado útil?

  3. Hi, Does it have any BSA in the medium? if so, what concentration?

    1 respuesta

    1. This medium contains Bovine Serum Albumin at 1000 mg/L.

      ¿Le ha resultado útil?

  4. Hi, can I know the composition of the media? Namely glucose, lactate and pyruvate is of best interest! Thanks

    1 respuesta

    1. Product MR-106 has the following composition:
      D-Glucose: 36.03 mg/L
      Sodium Lactate: 1121.00 mg/L
      Sodium Pyruvate: 22.00 mg/L

      ¿Le ha resultado útil?

  5. Hello, is it possible to send the KSOM just chilled and not frozen? We would prefer to get the media only on ice to make smaller aliquots so that we freeze it for the first time ourselves. 50ml is significant volume which we are not able to use in 2 weeks

    1 respuesta

    1. This product is shipped in dry ice at-20°C. Items requiring special storage conditions are stored according to the requirements indicated on the product-specific website or on the product label. Please read through Storage and Transport Conditions located on the "Quality &Regulatory" page of the website: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf

      ¿Le ha resultado útil?

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