Merck
All Photos(2)

A0168

Sigma-Aldrich

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody

Synonym(s):
Goat Anti-Mouse IgG (Fc specific)–HRP
Número MDL:
NACRES:
NA.46

Nivel de calidad

300

origen biológico

goat

conjugado

peroxidase conjugate

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

species reactivity

mouse

technique(s)

direct ELISA: 1:50,000
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

enviado en

dry ice

temp. de almacenamiento

−20°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Descripción general

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

Inmunógeno

purified mouse IgG Fc fragment

Aplicación

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in
  • western blot
  • enzyme linked immunosorbent assay (ELISA) and
  • indirect immunoperoxidase assay (IIPA)

Forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service