Merck
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A2304

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Goat Anti-Mouse IgG (Fab specific)- HRP
Número MDL:

Nivel de calidad

200

origen biológico

goat

conjugado

peroxidase conjugate

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

species reactivity

mouse

should not react with

human, horse, bovine

technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section
western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells

enviado en

dry ice

temp. de almacenamiento

−20°C

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Descripción general

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody associates with mouse IgG and mouse Fab fragment. The conjugate shows no reactivity with mouse Fc fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense and is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Aplicación

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)–Peroxidase antibody has been used in western blotting.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is suitable for use in immunohistochemistry applications at 1:200 dilution using human tonsil tissues. The antibody can also be used for western blot assays at 1:80,000-1:160,000 dilutions using total cell extract of HeLa cells.

Otras notas

Antibody adsorbed with bovine, equine, and human serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Nota de preparación

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Adsorbed to reduce background staining with bovine, horse and human samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 2

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