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Merck

A2356

Phosphatase, Alkaline from bovine intestinal mucosa

≥5,500 DEA units/mg protein

Phosphatase, Alkaline from bovine intestinal mucosa
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Sinónimos:

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Número CAS:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54
Número CE:
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849

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Nombre del producto

Phosphatase, Alkaline from bovine intestinal mucosa, ≥5,500 DEA units/mg protein

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

specific activity

≥5,500 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

storage temp.

2-8°C

Quality Level

200

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Phosphatase, Alkaline from bovine intestinal mucosa ≥5,500 DEA units/mg protein

Sigma-Aldrich

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Sigma-Aldrich

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Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, ≥5,700 DEA units/mg protein

Sigma-Aldrich

P0114

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein

Sigma-Aldrich

P7923

Phosphatase, Alkaline from bovine intestinal mucosa

specific activity

≥5,500 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

specific activity

≥5,700 DEA units/mg protein

specific activity

≥4,000 DEA units/mg protein

form

(Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

aqueous solution

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

buffered aqueous glycerol solution

concentration

≥10 mg/mL

concentration

≥1.0 mg/mL

concentration

≥10 mg/mL

concentration

≥10.0 mg/mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Analysis Note

Package sizes are based on DEA units

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000517861
0000517860
0000517861
0000517860
0000443598

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Protocolos

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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