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A3076

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

Name: Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal
Brand: SIGMA
Price: 232.05 EUR

clone GE-1, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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Talla/SKU	Disponibilidad	Precio
0.2 mL	Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	273,00 € 232,05 €
0.5 mL	Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad	548,00 € 465,80 €

Acerca de este artículo

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

GE-1, monoclonal

Application:

DB, ELISA (d)

Citations:

232,05 €

Precio de catálogo273,00 €Ahorre 15 %

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Propiedades

biological source

mouse

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Descripción

General description

Binds human IgE; does not bind human IgG, IgA or IgM.

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :

immunoblot assays
direct ELISA
dot blot

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Este artículo	A2064	A2831	A3312
Sigma-Aldrich A3076 Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich A2064 Anti-Human IgG−Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich A2831 Conjugado anti-DNP− monoclonal-fosfatasa alcalina antibody produced in mouse	Sigma-Aldrich A3312 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat
description clone GE-1, purified from hybridoma cell culture	description clone GG-5, purified from hybridoma cell culture	description clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
biological source mouse	biological source mouse	biological source mouse	biological source goat
species reactivity human	species reactivity human	species reactivity -	species reactivity human
technique(s) dot blot: 1:1,000, direct ELISA: 1:6,000	technique(s) western blot (chemiluminescent): 1:80,000, immunohistochemistry: suitable, direct ELISA: 1:50,000, dot blot: 1:80,000	technique(s) direct ELISA: 1:25,000 using DNP-BSA, dot blot: 1:180,000 (chemiluminescence)	technique(s) direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
isotype IgG2b	isotype IgG1	isotype IgE	isotype -

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Clase de almacenamiento

10 - Combustible liquids

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WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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