A3187

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Goat Anti-Human IgG (γ-chain specific)−AP
• Número MDL: MFCD00162443
• NACRES: NA.46

Properties

• origen biológico: goat
• Nivel de calidad: 200
• conjugado: alkaline phosphatase conjugate
• forma del anticuerpo: affinity isolated antibody
• antibody product type: secondary antibodies
• clon: polyclonal
• formulario: buffered aqueous glycerol solution
• species reactivity: human
• technique(s): direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C

Description

Descripción general

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific)-Alkaline Phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, and Bence Jones κ, and λ myeloma proteins.

Inmunógeno

Purified human IgG

Aplicación

Anti-Human IgG (γ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (at 1:2500 and 1:10,000 dilutions). The antibody can also be used for western blot (1:30,000) and immunohistochemistry (at 1:50 dilutions using formalin-fixed, paraffin-embedded sections).

Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).

Forma física

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Código de clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 2
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable