Merck
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A6535

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Synonym(s):
Adenosine 5′-triphosphatase, Adenosine 5′-diphosphatase
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

Nivel de calidad

300

tpo

Grade VII

formulario

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

composición

Protein, 25-60%

solubilidad

H2O: soluble 1.0 mg/mL

application(s)

diagnostic assay manufacturing

actividad extraña

Acid Phosphatase ≤2% of base activity

temp. de almacenamiento

−20°C

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Aplicación

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Envase

100, 200, 500, 1000, 2000 units in glass bottle
Sold on the basis of ATPase units.

Acciones bioquímicas o fisiológicas

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Otras notas

This grade has a low ATPase/ADPase ratio.

Definición de unidad

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Forma física

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Nota de preparación

Derived from red potato

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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