Merck
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A7646

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

Synonym(s):
Adenosine 5′-triphosphatase, Adenosine 5′-diphosphatase
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

origen biológico

potato

Nivel de calidad

200

tpo

Grade III

formulario

lyophilized powder (partially purified)

ATPase activity

≥60 units/mg protein

mol peso

~49 kDa by gel filtration

composición

Protein, 30-60%

solubilidad

H2O: soluble 10 mg/mL, slightly hazy

application(s)

diagnostic assay manufacturing

actividad extraña

Acid Phosphatase ≤5% of base activity

temp. de almacenamiento

−20°C

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Aplicación

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Envase

500, 1000, 2000, 5000 units in poly bottle
100, 200 units in glass bottle
Sold on the basis of ATPase units

Acciones bioquímicas o fisiológicas

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Otras notas

Mixture of high and low ratio isoenzymes.

Calidad

Contains ≤ 5% acid phosphatase.

Definición de unidad

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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