Although not validated, many users choose to aliquot the Accumax into single use vials after the initial thaw to store frozen at -20 °C until the expiration date.
A7089
Accumax™ solution
sterile-filtered, suitable for cell culture
Sinónimos:
Cell detachment solution
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| Tamaño de envase | SKU | Disponibilidad | Precio |
|---|---|---|---|
| 100 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 229,00 € | |
| 500 mL | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 735,00 € |
Acerca de este artículo
Concentration:
1 ×
229,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlesterility
sterile-filtered
Quality Level
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
Application
Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
TM=["Accumax"] solution has been used:
- for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
- for dissociation of retinal pigmented epithelial cells prior to passaging
- for dissociation of islets from pancreas
Biochem/physiol Actions
Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.
Features and Benefits
Ready-to-use sterile liquid for in vitro cell applications
Preparation Note
Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.
This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
Disclaimer
Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form solution | form liquid | form liquid | form solution |
| concentration 1 × | concentration 400-600 units/mL | concentration - | concentration 1 × |
| pH 6.8-7.8 | pH 6.8-7.8 | pH - | pH 7.0-9.0 |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile; sterile-filtered |
| storage temp. −20°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. −20°C |
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Artículos
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
Contenido relacionado
The PromoCell Primary Cancer Culture System allows selective isolation of malignant cancer cells from tumor associated stromal cells in patient derived xenographs (PDX models) and tumor samples.
Datasheet
Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells
Kaiser JP, et al.
Journal of King Saud University - Science, 25(1), 15-27 (2013)
Natalja Funk et al.
Scientific reports, 9(1), 4515-4515 (2019-03-16)
Mutations within Leucine-rich repeat kinase 2 (LRRK2) are associated with late-onset Parkinson's disease. The physiological function of LRRK2 and molecular mechanism underlying the pathogenic role of LRRK2 mutations remain uncertain. Here, we investigated the role of LRRK2 in intracellular signal
Benjamin T Dodsworth et al.
Scientific reports, 10(1), 10542-10542 (2020-07-01)
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| A7089-500ML | 04061833383490 |
| A7089-100ML | 04061833383483 |
-
After thawing Accumax, can I make aliquots and freeze them?
1 respuesta-
¿Le ha resultado útil?
-
-
Is your accumax gmp grade and made in a gmp compliant facility and is animal free?
1 respuesta-
This Accumax™ solution does not contain mammalian- or bacterial-derived products. It is not a GMP grade. This product is assigned a quality level of 200. Products with quality level of 200 are not suitable for GMP. Products with quality levels 400 or higher are suitable for GMP.
Currently, there is no GMP grade product available.Please see the quality brochure below for more information:
¿Le ha resultado útil?
-
-
What is the size of the bottle in size specifications (inches, centimeters)?
1 respuesta-
See below for the 100 mL and 500 mL bottle dimensions:
100 mL
Height: 104.1 mm
Height w/cap: 108.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 37.3 mm500 mL
Height: 173.2 mm
Height w/cap: 177.8 mm
Opening inner diameter: 28.4 mm
Outer thread diameter: 38 mm¿Le ha resultado útil?
-
-
What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
1 respuesta-
Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
¿Le ha resultado útil?
-
-
What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
¿Le ha resultado útil?
-