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Merck

C0543

Mouse Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Sinónimos:

Type IV collagen, EHS-collagen

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Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.75

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Nombre del producto

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

origen biológico

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Nivel de calidad

tpo

Type IV (Miller)

Línea del producto

BioReagent

Formulario

lyophilized powder

envase

glass bottle of 1 vial

técnicas

cell culture | mammalian: suitable

cobertura de la superficie

6‑10 μg/cm2

solubilidad

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Información sobre el gen

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Este artículo
C3867C7661C1188
biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

biological source

rat tail

biological source

rat tail

biological source

bovine cartilage (tracheal)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

liquid

form

powder

form

powder

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

-

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

solubility

soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)

solubility

soluble

solubility

aqueous acid: soluble

Descripción general

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma.[1] [2]The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Aplicación

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.[3]
  • in hydrogel fabrication.[4]
  • to coat cell culture plates for cell differentiation.[5]
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.[6]
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Acciones bioquímicas o fisiológicas

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules.[1] It is also used in 2D coatings.[7]

Nota de preparación

0.75 mg/vial
Chloroform treated.
Pepsin is not used in the preparation.

Otras notas

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Preguntas

  1. Could you provide me with the observed molecular weight of the Rat Collagen Type IV product from the Engelbreth-Holm-Swarm murine sarcoma basement membrane? CAS Number:9007-34-5

    1 respuesta
    1. The molecular weight for this product has not been determined in-house. Other sources have reported the molecular weight to be 484.7 g/mol.

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