C1907

Calcineurin from bovine brain

lyophilized powder, ≥2,500 units/mg protein

• Sinónimos: Calcium/Calmodulin-Activated Protein Phosphatase, Calmodulin binding protein, Modulator binding protein, PP2B, Phosphoprotein Phosphohydrolase, Protein phosphatase 2B
• Número CAS: 90371-51-0
• Número MDL: MFCD00130697
• Código UNSPSC: 51111800
• NACRES: NA.32

Propiedades

• origen biológico: bovine
• Nivel de calidad: 200
• Formulario: lyophilized powder
• actividad específica: ≥2,500 units/mg protein
• mol peso: dimer ~77 kDa; subunit mol wt 19-58 kDa
• composición: Protein, 0.3-1.7% Lowry
• solubilidad: H₂O: soluble
• Nº de acceso UniProt: P48452
• temp. de almacenamiento: −20°C
• Información sobre el gen: cow ... PPP3CA(286852)

Descripción

Descripción general

Calcineurin (CaN) comprises CaN A and CaN B subunits. It exists as a heterodimer with a calmodulin-binding domain, catalytic site, a CaN B binding domain, and an autoinhibitory domain.[1]

Aplicación

Calcineurin from bovine brain has been used:

• as a positive control in western blot analysis of oocytes and cumulus cells proteome[2]
• as a positive control in calmodulin (CaM)-agarose binding assay[3]
• to test its phosphatase activity in the presence of okadaic acid[4]

Acciones bioquímicas o fisiológicas

Calcineurin (CaN) activity is stimulated by nickel (Nⁱ²⁺) and manganese (Mn²⁺) ions.[1] It participates in the coupling of Ca²⁺ signals.[1] CaN may regulate oocyte growth and meiotic maturation in porcine.[2] It also participates in gene regulation, cell survival, and death.[4]

Calcineurin is a cyclosporin-sensitive, calcium-regulated, serine-threonine protein phosphatase with broad substrate specificity. It is the major calmodulin-binding protein found in the brain. First identified as an inhibitor of the calmodulin activation of phosphodiesterase 3′:5′ cyclic nucleotide (PDE), calcineurin has similar effects on adenylate cyclase. Serves as a key enzyme involved in T-cell activation. Also involved in the hyperphosphorylation of tau protein in Alzheimer′s disease and has been shown to prevent calpain-mediated proteolysis of tau in differentiated PC12 cells.

The major calmodulin-binding protein found in the brain. A key enzyme involved in T-cell activation and hyperphosphorylation of tau protein in Alzheimer′s disease.

Forma física

Lyophilized powder containing 0.5% EGTA, buffer salts and stabilizers.

Nota de análisis

Sigma tests activity in 80 mM Tris, pH 7.5 with 65 mM KCl, 8 mM MgSO₄ and 0.3% albumin.

Otras notas

One unit will cause a 50% inhibition of the activated phosphodiesterase, 3′:5′-cyclic nucleotide (P 9529) activity when assayed with two units of activator (P 2277) and 0.1 mM Ca²⁺ in an enzyme coupled system at pH 7.5 at 30 °C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves