Saltar al contenido
Merck

Saltar a

C2867

Citocromo c from equine heart

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

Sinónimos:

Citocromo c from horse heart

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
Talla/SKUDisponibilidadPrecio
10 mg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
265,00 €
50 mg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
802,00 €

Acerca de este artículo

Número CAS:
UNSPSC Code:
12352202
EC Number:
232-700-9
NACRES:
NA.25
MDL number:
Form:
powder
Assay:
≥99% (SDS-PAGE)
Biological source:
horse heart
Mol wt:
12,384

265,00 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

​
Solicita un pedido personalizado
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle


biological source

horse heart

Quality Segment

product line

BioUltra

assay

≥99% (SDS-PAGE)

form

powder

mol wt

12,384

storage condition

(Tightly closed Dry)

technique(s)

cell culture | mammalian: suitable

solubility

water: 10 mg/mL, dark red-brown

UniProt accession no.

storage temp.

−20°C

Gene Information

horse ... CYCS(100053958)

General description

Cytochrome C (Cyt C) is a nuclear DNA-encoded protein. It is derived from its precursor apo-cytochrome c and moves across the outer membrane of the mitochondria.

Research area: Apoptosis

Application

Cytochrome c from equine heart has been used to prepare a stock solution for synthesizing and calibrating biotinylated cytochrome c. It has also been used as a component of the reaction buffer to stain electron transport chain (ETC) complex IV and determine its activity using blue-native gel electrophoresis.
Se caracterizaron los sitios específicos y el grado de oxidación en el citocromo c caballo tratado con H2O2 y UV. Se sugirió que el estado de estos sitios podría utilizarse como biomarcador del entorno oxidativo de una célula.

Biochem/physiol Actions

Cytochrome C (Cyt C) acts as a potent signaling molecule of apoptosis by activating caspase 3, a death protease. It plays a role in heme-binding with the help of cytochrome c heme lyase which facilitates its release into the mitochondrial intermembrane space. Cyt C also exhibits lipid-binding by interacting with cardiolipin, a phospholipid that initiates protein unfolding and activates the peroxidase function in Cyt C.
El citocromo c se conoce fundamentalmente como una proteína mitocondrial transportadora de electrones. La transición del citocromo c entre los estados ferroso y férrico dentro de la célula la convierte en un eficiente transportador biológico de electrones y desempeña un papel vital en las oxidaciones celulares tanto en plantas como en animales. Se considera generalmente como un catalizador universal de la respiración, formando un puente de electrones esencial entre los sustratos respirables y el oxígeno

Preparation Note

A further purification of C2506
Prepared using TCA.

Other Notes


Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
C2506C7150C3131
description

BioUltra, ≥99% (SDS-PAGE), powder, suitable for mammalian cell culture

description

≥95% (SDS-PAGE)

description

BioReagent, suitable for GFC marker

description

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

biological source

horse heart

biological source

horse heart

biological source

-

biological source

bovine heart

technique(s)

cell culture | mammalian: suitable

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

assay

≥99% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

-

assay

≥95% based on Mol. Wt. 12,327 basis

form

powder

form

powder

form

powder

form

powder

mol wt

12,384

mol wt

12,384

mol wt

~12,400

mol wt

12327 Da

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Still not finding the right product?

Explore all of our products under Citocromo c from equine heart


Clase de almacenamiento

11 - Combustible Solids

¿Qué está pasando?

WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, type N95 (US)



Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos




Preguntas

1–5 de 5 Preguntas  
  1. I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?

    1 respuesta
    1. Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required.  Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system.  Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.

      ¿Le ha resultado útil?

  2. How should I store solutions of Product C2867, Cytochrome c?

    1 respuesta
    1. Our general recommendations are as follows:  Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.

      ¿Le ha resultado útil?

  3. How do I determine how much of Product C2867, Cytochrome c, is in the oxidized form, and how much is reduced?

    1 respuesta
    1. This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com).

      ¿Le ha resultado útil?

  4. What is the Department of Transportation shipping information for this product?

    1 respuesta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      ¿Le ha resultado útil?

  5. Is Product C2867, Cytochrome c, predominantly in the oxidized form or the reduced form?

    1 respuesta
    1. This product is predominantly (>95%) in the oxidized form.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos