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Biological Buffers

Light blue biological buffer solution in beaker with pipette.

It’s important to protect the integrity of your biomolecules and reagents by choosing the right biological buffer systems. We offer an extensive portfolio of biological buffers for a wide variety of applications, including cell culture buffers, PCR buffers, HEPES buffers, and assay buffers.

Key features of our advanced buffer systems:

  • High-purity solutions available in a wide range of grades
  • Multiple and user-friendly packaging options
  • Assured solution stability with pH control
  • No interference with biological processes
  • Add critical salts and nutrients to cells and tissues
  • Scalable from early research to commercial applications
  • Customized blending

Products

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TRISHEPESPBSSodium PhosphateMESMOPSBIS-TRISImidazole
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Dulbecco′s Phosphate Buffered Saline
D8537

Dulbecco′s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Phosphate buffered saline
P4417

Phosphate buffered saline

tablet, pH 7.2-7.6 (1 tablet/200 mL)

Trizma<SUP>®</SUP> base
T1503

Trizma® base

≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate

HEPES
H3375

HEPES

≥99.5% (titration)

Phosphate buffered saline
P3813

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

MES hydrate
M8250

MES hydrate

≥99.5% (titration)

HEPES
H4034

HEPES

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

HEPES solution
H0887

HEPES solution

1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent

Sodium bicarbonate
S5761

Sodium bicarbonate

powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture

Triethylammonium acetate buffer
90358

Triethylammonium acetate buffer

volatile buffer, ~1.0 M in H2O

RIPA Buffer
R0278

RIPA Buffer

Trizma<SUP>®</SUP> base
T6066

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Trizma<SUP>®</SUP> hydrochloride
T3253

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

MOPS
M1254

MOPS

≥99.5% (titration), crystalline powder

Ethylenediaminetetraacetic acid disodium salt dihydrate
E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, Molecular Biology, 99.0-101.0% (titration)

Imidazole
I202

Imidazole

ReagentPlus®, 99%

Dulbecco′s Phosphate Buffered Saline
D5652

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Dulbecco′s Phosphate Buffered Saline
D8662

Dulbecco′s Phosphate Buffered Saline

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

MES hydrate
M2933

MES hydrate

BioPerformance Certified, suitable for cell culture, ≥99.5%

Potassium phosphate monobasic
P5655

Potassium phosphate monobasic

powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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Our advanced buffering systems can bring phenomenal stability to your cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation-based applications. All our products can be scaled from early research to commercial applications.

Related Resources

  • Buffer Reference Center

    These tables will help you navigate the preparation of many common buffer solutions by pH and pKa. Choose a buffer with an effective pH range for your specific assay.

  • Polymerase Chain Reaction (PCR) Basics

    Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

  • TAE and TBE Running Buffers Recipe & Video

    TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

  • M-Clarity™ Program

    The MQ levels of the M-Clarity™ Program provide transparency so that you can choose, with confidence, suitable products that meet your needs.

  • Buffer Calculator

    Buffer solution calculator: Empirical formula, pKa, and buffer pH range calculations for various buffers.

  • Poster: Biological Buffers Selection Guide
  • Flyer: pHast Pack Electrophoresis

    The pHast Pack™ platform was developed to expedite your research and provide a convenient solution for tedious lab tasks.

  • Flyer: Tricine Buffer

    Our convenient Tricine high purity biological buffer was developed for unparalleled quality and economy.

  • Flyer: HEPES Buffer

    Our high-purity biological buffers will be HEPES of help for your research.

  • Flyer: Trizma Buffers

    Our convenient high purity Trizma® (tris) hydrochloride buffer and basimetric primary standard was developed for impeccable quality at a budget price.

  • Brochure: Biological Buffers

    High-purity biological buffers from bench to bulk volumes.

  • pHast Pack™ – Ready-to-Use Buffers

    Simplify your buffer solution preparation with our pHast Pack™ ready-to-use buffers which offer exact concentrations and pH without weighing, buffer calculations, and pH adjustments for reliable research results.

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Related Webinars

Slide 1 of 4
Webinar

HEPES Induces Lysosomes & Autophagy in Culture Cells

Discover how HEPES may influence cell culture experiments and what to be aware of in data interpretation.

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Novel viral therapies are emerging and gaining more interest after recent regulatory approval of CAR-T and AAV based gene therapies.

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In this webinar, you will learn how to process development for transient and stable producer cell lines.

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In this webinar, you will learn how to design a testing strategy to meet US FDA and EMA requirements.

Complete Workflow Solutions

We provide a comprehensive selection of high-quality biochemicals in a wide range of buffer grades and innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing and customized blending.

Bioperformance Certified Buffers

Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocessing workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include enzymatic assay of nuclease, protease, and phosphatase, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures the reproducibility of your experiment.

pHast Pack™ Ready-to-Use Buffers

Trying to get the correct concentration and pH value in buffer preparations can be a tedious and time-consuming task. Our pHast Pack™ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick and reproducible results again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.

Need help making a buffer? Our buffer preparation calculator helps you to quickly calculate buffer molarity, mass, and volume.

Related Videos

Slide 1 of 2
Improving Reproducibility with Biological Buffers
Improving Reproducibility with Biological Buffers
Watch this informative webinar in which our experts will explore ways to improve reproducibility when using biological buffers.
Redi-Dri™ – The World of Free-flowing Hygroscopic Products
Redi-Dri™ – The World of Free-flowing Hygroscopic Products
The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times.
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