Merck
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D1501

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from calf thymus

Type I, fibers

Synonym(s):
ctDNA, DNA sodium salt from calf thymus, Thymonucleic acid sodium salt
CAS Number:
Número MDL:
eCl@ss:
32160414
NACRES:
NA.51

tpo

Type I

Nivel de calidad

300

formulario

fibers

color

white

temp. de almacenamiento

2-8°C

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Aplicación

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Envase

50, 100, 500 mg in poly bottle
1, 5 g in poly bottle

Calidad

"Highly Polymerized"

Propiedades físicas

λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

Forma física

fibrous preparation

Nota de preparación

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.

Reconstitución

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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