E8008

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): EDTA solution
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₈
• CAS Number: 60-00-4
• Molecular Weight: 292.24
• Beilstein: 1716295
• Número MDL: MFCD00003541
• eCl@ss: 39030907
• ID de la sustancia en PubChem: 24894664
• NACRES: NA.75

Properties

• esterilidad: sterile-filtered
• Nivel de calidad: 200
• Línea del producto: BioReagent
• formulario: solution
• concentración: 0.02% in DPBS (0.5 mM)
• technique(s): cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• pH: 7.1-7.7
• enviado en: wet ice
• temp. de almacenamiento: 2-8°C
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

Descripción general

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Aplicación

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Forma física

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Safety Information

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• WGK: nwg
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable