F5259

Anti-Human IgA (α-chain specific)−FITC antibody produced in goat

Name: Anti-Human IgA (α-chain specific)−FITC antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Human IgA (α-chain specific)−Fluorescein isothiocyanate

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UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162415

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d)

Citations:

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biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:16

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

200

Categorías relacionadas

Secondary Antibodies

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Este artículo	F9512	F3761	A6907
Sigma-Aldrich F5259 Anti-Human IgA (α-chain specific)−FITC antibody produced in goat	Sigma-Aldrich F9512 Anti-Human IgG (Fc specific)−FITC antibody produced in goat	Sigma-Aldrich F3761 Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody produced in goat	Sigma-Aldrich A6907 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) direct immunofluorescence: 1:16	technique(s) immunofluorescence: 1:64-1:128 using Hep2 cells	technique(s) direct immunofluorescence: 1:16	technique(s) direct ELISA: 1:10,000
biological source goat	biological source goat	biological source goat	biological source goat
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate peroxidase conjugate
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

General description

IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces
Anti-Human IgA (α-chain specific)-FITC antibody is specific for α chain of human IgA. Goat Anti-Human IgA is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified human IgA

Application

Anti-Human IgA (α-chain specific)-FITC antibody may be used for direct immunofluorescence at a working antibody dilution of 1:16 to 1:32. A dilution of 1:100 was used for immunohistochemistry of cryosections of human sural nerves.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Local activation of the complement system in endoneurial microvessels of diabetic neuropathy.

G B Rosoklija et al.

Acta neuropathologica, 99(1), 55-62 (2000-01-29)

Quantitative immunocytochemical analysis of complement proteins (CP) was performed on sural nerve biopsies from 15 patients with diabetic neuropathy (DN) and 18 nondiabetic patients with other forms of chronic neuropathy (ON). The mean age of the patients and the pathological

Cyclodextrin Reduces Cholesterol Crystal-Induced Inflammation by Modulating Complement Activation.

Siril S Bakke et al.

Journal of immunology (Baltimore, Md. : 1950), 199(8), 2910-2920 (2017-09-01)

Cholesterol crystals (CC) are abundant in atherosclerotic plaques and promote inflammatory responses via the complement system and inflammasome activation. Cyclic oligosaccharide 2-hydroxypropyl-β-cyclodextrin (BCD) is a compound that solubilizes lipophilic substances. Recently we have shown that BCD has an anti-inflammatory effect

Histone deacetylase inhibitors upregulate B cell microRNAs that silence AID and Blimp-1 expression for epigenetic modulation of antibody and autoantibody responses.

Clayton A White et al.

Journal of immunology (Baltimore, Md. : 1950), 193(12), 5933-5950 (2014-11-14)

Class-switch DNA recombination (CSR) and somatic hypermutation (SHM), which require activation-induced cytidine deaminase (AID), and plasma cell differentiation, which requires B lymphocyte-induced maturation protein-1 (Blimp-1), are critical for the generation of class-switched and hypermutated (mature) Ab and autoantibody responses. We

B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids.

Helia N Sanchez et al.

Nature communications, 11(1), 60-60 (2020-01-04)

Short-chain fatty acids (SCFAs) butyrate and propionate are metabolites from dietary fiber's fermentation by gut microbiota that can affect differentiation or functions of T cells, macrophages and dendritic cells. We show here that at low doses these SCFAs directly impact B

A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease.

Valeria R Villella et al.

The EMBO journal, 38(2) (2018-12-01)

Intestinal handling of dietary proteins usually prevents local inflammatory and immune responses and promotes oral tolerance. However, in ~ 1% of the world population, gluten proteins from wheat and related cereals trigger an HLA DQ2/8-restricted TH1 immune and antibody response leading

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