F7759

Sigma-Aldrich

Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Número MDL:
NACRES:
NA.46

origen biológico

rabbit

Nivel de calidad

200

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

aplicaciones

direct immunofluorescence: suitable

conjugado

FITC conjugate

temp. de almacenamiento

−20°C

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Descripción general

Horse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in horse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Horse IgG has different subclasses, IgG2a, IgG2b, IgG2c, IgG1 and IgG(T). Compared to other IgG molecules, IgG(T) has more carbohydrate content. In healthy individuals, total IgG is dispersed equally between circulating plasma and interstitial fluids.

Especificidad

Anti-horse IgG (whole molecule)-FITC antibody is specific for horse IgG subclasses.

Inmunógeno

Purified horse IgG

Aplicación

Anti-Horse IgG (whole molecule) FITC antibody produced in rabbit has been used in indirect fluorescent antibody test. It has also been used as a secondary antibody to confirm equine arteritis virus (EAV).
Rabbit polyclonal anti-Horse IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in horse serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use horse IgG as the primary antibody.

Acciones bioquímicas o fisiológicas

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense.
IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B- cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta that is vital for immune defense of the neonate against infections.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

RIDADR

NONH for all modes of transport

WGK Alemania

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

A 34-year retrospective study of equine viral abortion in Poland
Bazanow BA, et al.
Polish Journal of Veterinary Sciences, 17(4), 607-612 (2014)
Advances in Immunology (1964)
Encyclopedia of Immunology (1998)
Alexandre Dias Munhoz et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 28(2), 283-290 (2019-06-20)
The aim of this study was to determine the frequency and factors associated with Toxoplasma gondii in naturally infected equids in northeastern Brazil. Serum samples from 569 equids (528 horses, 8 mules and 33 donkeys) were subjected to the indirect...
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