Merck

F9512

Sigma-Aldrich

Anti-Human IgG (Fc specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Sinónimos:
Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat, Anti Human Igg Fitc
Número MDL:
NACRES:
NA.46

origen biológico

goat

conjugado

FITC conjugate

forma del anticuerpo

affinity isolated antibody

antibody product type

secondary antibodies

clon

polyclonal

formulario

buffered aqueous solution

condiciones de almacenamiento

protect from light

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

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Este artículo
F5512F0132F3512
conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

technique(s)

immunofluorescence: 1:16-1:32 using Hep2 cells

technique(s)

direct immunofluorescence: 1:32

technique(s)

immunofluorescence: 1:32-1:64 using Hep2 cells

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Descripción general

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG.

Especificidad

Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples

Inmunógeno

Purified human IgG Fc fragment

Aplicación

Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in:
  • immunofluorescence
  • immunohistochemistry
  • surface labeling experiments

Acciones bioquímicas o fisiológicas

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense.

Otras notas

No cross-reaction with mouse and rat IgG.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg


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