The lyophilized powder may be reconstituted at 5 mg/mL in 0.02M sodium phoshate buffer or 0.45% sodium chloride containing 0.01% BSA. Solutions prepared at concentrations of at least 1 mg/ml may be stored in aliquots at -20°C for several months.
H2126
Hyaluronidase from sheep testes
Type II, lyophilized powder, ≥300 units/mg solid
Sinónimos:
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
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Servicio técnico
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Permítanos ayudarleNombre del producto
Hyaluronidase from sheep testes, Type II, lyophilized powder, ≥300 units/mg solid
conjugate
conjugate (Glucosaminoglycan)
type
Type II
form
lyophilized powder
specific activity
≥300 units/mg solid
mol wt
55 kDa
storage temp.
−20°C
Quality Level
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Este artículo | HX0514 | H6254 |
|---|---|---|
| specific activity ≥300 units/mg solid | specific activity ≥300 units/mg dry wt. | specific activity ≥1,500 units/mg solid |
| form lyophilized powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 55 kDa | mol wt - | mol wt 55 kDa |
| Quality Level 200 | Quality Level 100 | Quality Level 200 |
| conjugate conjugate (Glucosaminoglycan) | conjugate conjugate (Glucosaminoglycan) | conjugate - |
Application
Hyaluronidase from sheep testes has been used:
- in flow cytometry[1]
- in the isolation of putative spermatogonial stem cells (SSCs)[2]
- to study the effects of magnetic stimulation in magnetic hydrogel properties[3]
- in immunohistochemistry to visualize collagen type II distribution[4][5]
- to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings[6]
- for the degeneration of hydrogels[7]
Biochem/physiol Actions
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
General description
Hyaluronidase is a hyaluronic acid-metabolizing enzyme.[8] It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.[9]
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Physical form
Lyophilized powder containing lactose
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity
Poldervaart MT, et al.
PLoS ONE, 12(6), e0177628-e0177628 (2017)
The role of the mechanotransduction ion channel candidate Nanchung-Inactive in auditory transduction in an insect ear.
Warren B and Matheson T
The Journal of Neuroscience, 2310-2317 (2018)
Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting
Mouser VHM, et al.
Biofabrication, 8(3), 035003-035003 (2016)
Robert B Struijk et al.
PloS one, 15(3), e0230253-e0230253 (2020-03-17)
Autologous transplantation of spermatogonial stem cells is a promising new avenue to restore fertility in infertile recipients. Expansion of the initial spermatogonial stem cell pool through cell culturing is a necessary step to obtain enough cells for effective repopulation of
Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture
Otto IA, et al.
European Cells & Materials, 35, 132-150 (2018)
Artículos
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocolos
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
Contenido relacionado
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Kann die Hyaluronidase H2126 in PBS gelöst werden um sie dann in kleinen Aliquoten bei -20 C zu lagern?
1 respuesta-
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H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。
1 respuesta-
This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparationPlease see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdf¿Le ha resultado útil?
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