Lysosome-associated membrane protein 1 (LAMP1), also termed Igp120, is a heavily glycosylated lysosomal membrane protein of about 120 kDa. It consists of a ~40 kDa core polypeptide with O-linked and 18 asparagine-linked oligosaccharide side chains. LAMP1 protein contains a leader sequence, a large intralumenal region consisting of 2 homologous domains separated by a hinge region rich in proline and serine, a 24-amino acid transmembrane region, and a short cytoplasmic tail containing the lysosomal membrane targeting signal. LAMP1 is ubiquitously expressed and highly conserved. It localizes mainly to lysosomes although a small portion is detected on the cell surface. It was found that highly metastatic tumor cells express more LAMP molecules on the cell surface than poorly metastatic cells.
The gene LAMP1 (lysosomal-associated membrane protein 1) encodes a type I transmembrane protein that has a short cytoplasmic tail containing a lysosome-targeting signal of GYQTI(382)-COOH. The gene is mapped to human chromosome 13q34.
synthetic peptide corresponding to amino acid residues 405-416 of human LAMP1 with N-terminal added cysteine-glycine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Anti-LAMP1-Cy3® antibody produced in rabbit has been used in western blotting and immunofluorescence.
Acciones bioquímicas o fisiológicas
Lysosome-associated membrane protein 1 (LAMP1) with heavy glycosylation, may be important to protect the lysosomal membrane from proteolytic enzymes within lysosomes.
The gene LAMP1 (lysosomal associated membrane protein 1) encodes a membrane glycoprotein that functions as an intracellular receptor. It is found to be expressed in the cytoplasm of several types of tumor cells and may be involved in tumor invasion. Lamp1 is crucial for perforin trafficking to the lytic granules and motility of these lytic granules. Its knockdown leads to inhibition of cytotoxicity of human natural killer cells.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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