2-Mercaptoethanol is compatible with a wide range of common aqueous buffers, such as Laemmli sample buffer, phosphate-buffered saline, or Tris buffer. Certain buffers, such as Tris, can cause oxidation of 2-mercaptoethanol. In these situations, preparation of the solution is recommended just before use.
M3148
2- Mercaptoetanol
Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
Sinónimos:
β-Mercaptoetanol, 2- Hidroxietilmercaptano, BME, Tioetilenglicol
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25 ML
24,10 €
100 ML
60,00 €
250 ML
132,00 €
500 ML
208,00 €
Acerca de este artículo
Fórmula lineal:
HSCH2CH2OH
Número CAS:
Peso molecular:
78.13
Beilstein:
773648
Número CE:
Número MDL:
Código UNSPSC:
12161504
ID de la sustancia en PubChem:
NACRES:
NA.31
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Molecular Biology
densidad de vapor
2.69 (vs air)
presión de vapor
1 mmHg ( 20 °C)
Línea del producto
BioReagent
Ensayo
99% (GC/titration)
Formulario
liquid
lim. expl.
18 %
idoneidad de la reacción
reagent type: reductant
concentración
14.3 M (pure liquid)
técnicas
cell culture | mammalian: suitable
electrophoresis: suitable
índice de refracción
n20/D 1.500 (lit.)
pH
4.5-6 (20 °C, 500 g/L)
bp
157 °C (lit.)
solubilidad
H2O: soluble 1 mL/mL
densidad
1.114 g/mL at 25 °C (lit.)
actividad extraña
DNase, RNase, protease, none detected
temp. de almacenamiento
2-8°C
cadena SMILES
OCCS
InChI
1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
Clave InChI
DGVVWUTYPXICAM-UHFFFAOYSA-N
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Este artículo | 63689 | 8.05740 | M6250 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| technique(s) cell culture | mammalian: suitable, electrophoresis: suitable | technique(s) RNA extraction: suitable, protein extraction: suitable | technique(s) - | technique(s) - |
| assay 99% (GC/titration) | assay ≥99.0% (GC) | assay ≥99% (GC) | assay ≥99.0% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioReagent | product line BioUltra | product line - | product line - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C | storage temp. 2-8°C |
Aplicación
Agente reductor
βEl β-mercaptoetanol o 2-mercaptoetanol se ha utilizado:
βEl β-mercaptoetanol o 2-mercaptoetanol se ha utilizado:
BME es idóneo para reducir los puentes disulfuro de las proteínas antes de la electroforesis en gel de poliacrilamida y suele incluirse en un tampón de muestra para SDS-PAGE a una concentración de un 5 %. La escisión de los puentes disulfuro intermoleculares (entre subunidades) permite que las subunidades de una proteína se separen de forma independiente en la SDS-PAGE. La escisión de los puentes disulfuro intramoleculares (dentro de la subunidad) permite la desnaturalización completa de las subunidades para que cada péptido migre en función de la longitud de su cadena sin influencia de la estructura secundaria.
Palabra de señalización
Danger
Frases de peligro
Clasificaciones de peligro
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
Órganos de actuación
Liver,Heart
Código de clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
154.4 °F - closed cup
Punto de inflamabilidad (°C)
68 °C - closed cup
Equipo de protección personal
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Protocolos
Protocolo del ensayo con MTT para medir la viabilidad, la proliferación y la citotoxicidad celulares. Instrucciones para la preparación de los reactivos MTT y ejemplos de aplicaciones.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
Protocole du test au MTT pour la mesure de la viabilité, de la prolifération et de la toxicité cellulaires. Instructions de préparation du réactif au MTT et exemples d'applications.
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In which buffer or solvent should 2-Mercaptoethanol be diluted for working solution preparation?
1 respuesta-
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How can I determine the shelf life / expiration / retest date of this product?
1 respuesta-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf¿Le ha resultado útil?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 respuesta-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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I’ve purchased 25mL bottle and want to use this in my cell culture media at concentration of 50mM. What is the concentration in the 25mL bottle and how to make this to 50mM concentration?
1 respuesta-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
To prepare a 50 mM solution, please mix 0.391 g of M3148 with 100 mL solvent. 0.391 g / 78.13 g/mol / 0.1 L = 0.05 = 50 mM.¿Le ha resultado útil?
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Hi, What is the concentration of 2-mercaptoethanol in the 100mL bottle?
1 respuesta-
This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
¿Le ha resultado útil?
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Hi is this sterile?
1 respuesta-
This product is not sterile, but can be used in cell culture applications and is DNase, RNase, and protease free.
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