R7876
Ribonucleic acid, transfer from wheat germ
Type V, 15-19 units/mg solid
Sinónimos:
Transfer RNA, tRNA
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Molecular Biology
Nivel de calidad
tpo
Type V
Formulario
lyophilized powder
actividad específica
15-19 units/mg solid
condiciones de almacenamiento
desiccated
solubilidad
H2O: ~2 mg/mL, clear to hazy
temp. de almacenamiento
2-8°C
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Este artículo | R4752 | R8508 | R5636 |
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form buffered aqueous solution | form buffered aqueous solution |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: ~2 mg/mL, clear to hazy | solubility - | solubility - | solubility - |
| storage condition desiccated | storage condition - | storage condition - | storage condition - |
| grade for molecular biology | grade - | grade for molecular biology | grade for molecular biology |
Descripción general
Wheat germ tRNA contains many different specific tRNA molecules.
Aplicación
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Nota de preparación
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Otras notas
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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