SAB4200598

Monoclonal Anti-FTH1 antibody produced in mouse

~1.0 mg/mL, clone FTH24, purified immunoglobulin

• Sinónimos: Monoclonal Anti-FHC, Monoclonal Anti-FTH, Monoclonal Anti-FTH1, Monoclonal Anti-FTHL6, Monoclonal Anti-OK/SW-cl.84, Monoclonal Anti-PIG15, Monoclonal Anti-PLIF, Monoclonal Anti-cell proliferation-inducing gene 15 protein, Monoclonal Anti-ferritin H subunit, Monoclonal Anti-ferritin heavy chain, Monoclonal Anti-ferritin, heavy polypeptide 1, Monoclonal Anti-placenta immunoregulatory factor, Monoclonal Anti-proliferation-inducing protein 15
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: FTH24, monoclonal
• Formulario: buffered aqueous solution
• reactividad de especies: human
• concentración: ~1.0 mg/mL
• técnicas: flow cytometry: 10-20 μg/test using HeLa cells; immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa).; indirect immunofluorescence: 5-10 μg/mL using HeLa cells
• isotipo: IgG1
• Nº de acceso UniProt: P02794
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FTH1(2495)

Descripción

Descripción general

Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas.

Inmunógeno

synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Aplicación

Monoclonal Anti-FTH1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• flow cytometry
• immunofluorescence

Acciones bioquímicas o fisiológicas

Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer.

Monoclonal Anti- FTH1 recognizes human FTH1.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable