SAE0088

Filipin complex ready made solution

from Streptomyces filipinensis, 5mg/ml in DMSO based solution

• Sinónimos: Filipin solution
• NACRES: NA.77
• UNSPSC Code: 12352200

Propiedades

• Nombre del producto: Filipin complex ready made solution, from Streptomyces filipinensis, 5mg/ml in DMSO based solution
• assay: ≥90% (HPLC)
• form: ready-to-use solution
• storage condition: protect from light
• shipped in: dry ice
• storage temp.: −20°C

Descripción

Application

Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.[1]

Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.

Biochem/physiol Actions

Filipin is an antibiotic with antifungal functionality.[2] Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol.[3] On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.[2]

General description

Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.[3]

Other Notes

Filipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup