SRP0695

TAU-441, TTBK1-Phosphorylated

recombinant

• Sinónimos: Phosphorylated TAU antibody
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• recombinante: expressed in E. coli
• Ensayo: 70-95% (SDS-PAGE)
• Formulario: frozen liquid
• mol peso: ~64-70 kDa
• concentración: 0.2 μg/μL
• nº de acceso: P10636-8
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... MAPT(4137)

Descripción

Aplicación

Useful for kinase assay and Western blot.

Acciones bioquímicas o fisiológicas

Recombinant human tag-free Tau-441 was co-expressed with TTBK1 in E. coli cells. The Tau-441 was phosphorylated by TTBK1 in vivo and in vitro prior to the final chromatography purification. Tau-441 or Tau-F is a member of the Tau family of proteins which function to stabilize the microtubules by binding to them. Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules ⁽¹⁾. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, β-amyloid precursor protein (βAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure. Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system ⁽²⁾.

Nota de preparación

Formulated in 50mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable