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Merck

T0565

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

Sinónimos:

TMB membrane substrate

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UNSPSC Code:
12352204
NACRES:
NA.83

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Nombre del producto

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, ready to use solution

form

liquid

storage temp.

2-8°C

Quality Level

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Este artículo
T0440T9455T5569
Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

-

form

liquid

form

liquid

form

solution

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used as a chromogenic substrate in western blot analysis. It has also been used as a liquid substrate in enzyme-linked immunosorbent assay (ELISA).
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay[1]
  • as a substrate for IgG peroxidase[2]
  • in the visualization of immunocomplexes[3]

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.[4]

Physical form

Ready-to-use.

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus
Berdun S, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(10), G801-G806 (2015)
Navilla Apú et al.
Open life sciences, 18(1), 20220577-20220577 (2023-08-17)
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use enzyme-linked immunosorbent assays (ELISA) or chemiluminescence immunoassays (CLIA); however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests
Hesham Saeed et al.
Protein expression and purification, 181, 105820-105820 (2021-01-14)
In previous studies Pseudomonas aeruginosal-ASNase complete coding sequence gene, 984 bp (GenBank accession number KU161101.2) was isolated by PCR, cloned into pET28a(+) vector, expressed in E. coli DE3(BL21) pLysS, purified to apparent homogeneity and biochemically characterized. In the present work
Hesham Saeed et al.
Preparative biochemistry & biotechnology, 52(6), 668-680 (2021-10-07)
Microbial L-asparaginases are aminohydrolases that hydrolyze L-asparagine to L-aspartate. They are used to treat acute lymphoblastic leukemia and Hodgkin's lymphomas and in food industries. Increasing demand for L-ASNases is therefore needed. In the current study, the recombinant L-ASNase from Dickeya
immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
Rascon-Castelo E, et al.
Vaccines, 3(4), 973-987 (2015)

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This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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