Y1876

Yeast Synthetic Drop-out Medium Supplements

without tryptophan

• Sinónimos: Yeast Growth Medium
• UNSPSC Code: 12352200
• NACRES: ND.02
• MDL number: MFCD01095679

Propiedades

• Nombre del producto: Yeast Synthetic Drop-out Medium Supplements, without tryptophan
• form: powder
• application(s): food and beverages
• Quality Level: 100

Descripción

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for tryptophan. All the amino acids are present at a concentration of 76 mg/L except for leucine, which is present at a concentration of 380 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).

Preparation Note

1.92 g will supplement 1 liter of medium.

Información de seguridad

• Clase de almacenamiento: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)