Panax notoginseng attenuates experimental colitis in the azoxymethane/dextran sulfate sodium mouse model.

Phytotherapy research : PTR (2013-10-22)
Xiao-Dong Wen, Chong-Zhi Wang, Chunhao Yu, Lei Zhao, Zhiyu Zhang, Adiba Matin, Yunwei Wang, Ping Li, Shu-Yuan Xiao, Wei Du, Tong-Chuan He, Chun-Su Yuan
RESUMEN

Patients suffering from inflammatory bowel disease are at a high risk of developing colorectal cancer. To assess the anticancer potential of botanicals, in this study, we evaluated the effects of Panax notoginseng on azoxymethane/dextran sulfate sodium (DSS)-induced colitis. One week after A/J mice received azoxymethane, the animals received DSS for 8 days or were supplemented with P. notoginseng extract, at 30 or 90 mg/kg. DSS-induced colitis was scored with the disease activity index. The severity of the inflammatory lesions was evaluated by a colon tissue histological assessment. The expression of inducible nitric oxide synthase and cyclooxygenase-2 (COX-2) were also explored. We observed that the effects of P. notoginseng on the reduction of colon inflammation, expressed in disease activity index score, were in a dose-related manner (p < 0.01). P. notoginseng inhibited the reduction of the colon length and the loss of bodyweight in dose-related manner (all p < 0.05). The histological assessment of the colitis and inflammatory-related immunohistochemical data also supported the pharmacological observations. Our data suggest that P. notoginseng is a promising candidate in preventing and treating colitis and inflammation-associated colon carcinogenesis.

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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
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Dimethyl sulfoxide, for molecular biology
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Ethanol, anhydrous, denatured
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Dimethyl sulfoxide, anhydrous, ≥99.9%
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Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
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Ethanol, for residue analysis
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Ethanol, standard for GC
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Acetonitrile, anhydrous, 99.8%
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Ethanol, purum, secunda spirit, denaturated with 2% 2-butanone and 0.5% 4-methyl-2-pentanone, S15, ~96% (based on denaturant-free substance)
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Dimethyl sulfoxide, ACS reagent, ≥99.9%
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Ethanol, tested according to Ph. Eur.
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Acetonitrile, HPLC Plus, ≥99.9%
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Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
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Sodium sulfate, ACS reagent, ≥99.0%, anhydrous, granular
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Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
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Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
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Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
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Sodium sulfate, ACS reagent, ≥99.0%, anhydrous, powder
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1-Butanol, anhydrous, 99.8%
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Acetonitrile, ACS reagent, ≥99.5%
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1-Butanol, 99.9%
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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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1-Butanol, ACS reagent, ≥99.4%
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Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
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Azoxymethane, 13.4 M, ≥98%
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1-Butanol, for molecular biology, ≥99%
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1-Butanol, suitable for HPLC, ≥99.7%
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Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)