Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate.

Tetra-nickel substituted polyoxotungsate as an efficient sorbent for the isolation of His6-tagged proteins from cell lysate.

Talanta (2017-05-30)

Meng-Meng Wang, Qing Chen, Dan-Dan Zhang, Xu-Wei Chen, Ming-Li Chen

RESUMEN

By virtue of the flexible structure of polyoxometalates, Ni

MATERIALES

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Sigma-Aldrich

Albúmina from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

Citocromo c from equine heart, ≥95% (SDS-PAGE)

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Hemoglobin from bovine blood, suitable for protease substrate, substrate powder

Sigma-Aldrich

Myoglobin from equine heart, ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

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α-Lactalbumin from bovine milk, Type I, ≥85% (PAGE), lyophilized powder

Sigma-Aldrich

Lisozima from chicken egg white, dialyzed, lyophilized, powder, ~100000 U/mg