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Importance of neonatal immunoglobulin transfer for hippocampal development and behaviour in the newborn pig.

PloS one (2017-06-29)
Kateryna Goncharova, Liudmyla Lozinska, Ester Arevalo Sureda, Jarosław Woliński, Björn Weström, Stefan Pierzynowski
ABSTRACT

Neurological disorders are among the main clinical problems affecting preterm children and often result in the development of communication and learning disabilities later in life. Several factors are of importance for brain development, however the role of immunoglobulins (passive immunity transfer) has not yet been investigated. Piglets are born agammaglobulinemic, as a result of the lack of transfer of maternal immunoglobulins in utero, thus, they serve as an ideal model to mimic the condition of immunoglobulin deficiency in preterm infants. Thirty six, unsuckled newborn piglets were fed an infant formula or colostrum and supplemented orally or intravenously with either species-specific or foreign immunoglobulin and then compared to both newborn and sow-reared piglets. Two days after the piglets were born behavioural tests (novel recognition and olfactory discrimination of conspecifics scent) were performed, after which the piglets were sacrificed and blood, cerebrospinal fluid and hippocampi samples were collected for analyses. Both parameters of neuronal plasticity (neuronal maturation and synapse-associated proteins) and behavioural test parameters appeared to be improved by the appearance of species-specific porcine immunoglulin in the circulation and cerebrospinal fluid of the piglets. In conclusion, we postulate possible positive clinical effects following intravenous infusion of human immunoglobulin in terms of neuronal plasticity and cognitive function in preterm infants born with low blood immunoglobulin levels.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Bovine IgG (whole molecule) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
IgG from bovine serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sigma-Aldrich
Anti-Pig IgG (whole molecule) antibody produced in rabbit, whole antiserum
Sigma-Aldrich
IgG from porcine serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder