03-0167-00

SMC^® Human IL-1α High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-1α in human serum and plasma

• Synonym(s): IL-1 alpha Single Molecule Counting Kit, IL-1 alpha immunoassay kit, IL-1 alpha ultra-sensitive immunoassay, Interleukin-1 alpha immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• manufacturer/tradename: SMC^®
• technique(s): single molecule counting: suitable
• input: serum; plasma (K2 EDTA)
• UniProt accession no.: P01583
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments (validated)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Interleukin-1 alpha (IL-1α) is a pro-inflammatory cytokine produced by various cell types, including macrophages, fibroblasts, and epithelial cells, and plays a key role in the immune response. It is involved in the regulation of inflammation, cell proliferation, and apoptosis, acting as a crucial mediator in the body′s response to infection and injury. IL-1α is also implicated in the pathogenesis of several inflammatory diseases, highlighting its importance in both protective immunity and pathological conditions.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Biochem/physiol Actions

Although IL-1α was detectablein non-human primate, canine, and rat plasma, the assay has not been verified for these species.

Features and Benefits

• Exceptional Detection Limits: With detection capabilities in the femtogram/mL range, this immunoassay excels in identifying low concentrations of IL-1α, making it invaluable for researchers focused on early disease detection.
• Accurate Biomarker Monitoring: The high sensitivity of the kit enables precise monitoring of IL-1α levels, essential for understanding inflammatory pathways and assessing treatment responses in research settings focused on conditions like rheumatoid arthritis and sepsis.
• Versatile Research Applications: Designed for a variety of research environments, this ultrasensitive assay kit is ideal for applications in drug development, biomarker discovery, and immunological studies, enhancing its relevance across multiple scientific disciplines.
• Improved Experimental Outcomes: By facilitating early detection and effective monitoring of inflammatory responses, this kit contributes to more robust experimental designs and findings, ultimately leading to better insights into disease mechanisms.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 120 fg/mL* (SMCxPRO^® Instrument
• Standard Curve Range: 60-60,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 109% Plasma: 109%
• Recovery: Serum: 93% Plasma: 93%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Inflammation & ImmunologyInflammatory DiseaseImmune ResponseAutoimmune Disease

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H319,H360FD,H412
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects