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X-tremeGENE HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

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Synonym(s):
Xtremegene, transfection, transfection reagent

grade

for molecular biology

Quality Level

form

liquid (aqueous solution)

usage

 mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

−20°C

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GeneJuice® Transfection Reagent

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

packaging

pkg of 0.4 mL (06366244001), pkg of 5 × 1 mL (06366546001), pkg of 1.0 mL (06366236001)

packaging

pkg of 0.4 mL (06365779001), pkg of 1.0 mL (06365787001), pkg of 5 × 1.0 mL (06365809001)

packaging

pkg of 1 mL (04476093001 [1 mg/ml]), pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

packaging

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid (aqueous solution)

form

liquid (aqueous solution)

form

-

form

liquid

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

  • Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
  • Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
  • Generate physiologically relevant data using a reagent with low cytotoxic effects.
  • Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

Quality

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

334.4 °F

Flash Point(C)

168 °C


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Articles

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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Protocols

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

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