WISH (HeLa derivative)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human cervix
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human cervix
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 88102403-DNA-5UG, pkg of vial of cells 88102403-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Virus studies: VSV (Indiana), Ad 3, Poliovirus, measles virus

Biochem/physiol Actions

Human cervix carcinoma

Originally derived from human amnion tissue. Have been used in virus studies; susceptible to VSV (Indiana), adenovirus 3 and poliovirus. Useful in differentiating virulent and avirulent measles virus. HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered as derived from HeLa. Ethnicity: Black.

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:10 i.e. seeding at 1-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.