Generating and isolating high-producing cell lines or clones can be costly and labor-intensive. Instead, free your R&D to focus on developing new therapeutics by outsourcing the engineering of production cell lines to our CHOZN® Platform Development Team of highly trained scientists.
We can support your biomanufacturing and research with years of expertise in genome editing for Chinese hamster ovary (CHO) cell lines. Our customized services use a variety of molecular biology methods, including our own CompoZr® zinc finger nuclease (ZFN) technology, to deliver efficient cell lines that help reduce product costs and timelines.
Each cell line engineering project is divided into phases, invoiced only after a phase has been completed. We stay in close technical communication with your R&D team so that projects progress smoothly, advancing to each new phase when there is mutual agreement.
To discuss your needs, simply contact our team and a scientist will get in touch. We will then prepare a proposal detailing your project, which can begin with your approval.
A powerful tool for creating novel CHO lines, ZFNs are a class of engineered DNA-binding proteins that create double-strand breaks in DNA at user-specified locations. These double-strand breaks stimulate the cell's natural DNA-repair processes via homologous recombination (HR) and non-homologous end joining (NHEJ). By applying well-established and robust protocols, our team can make targeted genome edits such as:
We offer ZFN reagents in DNA plasmid format, as well as mRNA. Still another option is fluorescently tagged ZFNs, enabling fluorescence-activated cell sorting (FACS) that can result in an enriched pool of desired cells.