Alicyclobacillus spp. can be difficult to control in fruit juice products as their spores survive juice pasteurization temperatures and may subsequently germinate and grow after processing if conditions are suitable. The economic impact of such incidents can be very high. The International Fruit and Vegetable Juice Association (IFU) published a revised Method No. 12 standard for detection and enumeration of spore-forming, thermo-acidophilic bacteria (Alicyclobacillus spp.) in 2019.
This third edition from 2019 cancels and replaces the second edition of IFU Method No. 12: 2007, which has been technically revised. The main changes introduced in the third version, compared to IFU Method No. 12:2007, are considered as major. The IFU method no. 12 is now more precise, more structured, and therefore more reproducible. It includes improved culture media formulations and performance criteria which are in agreement with ISO procedures.
An ISO-compliant validation within the IFU Microbiological Working Group was performed, ensuring a user-friendly laboratory implementation. The main changes, compared to IFU method no. 12:2007, will be the following
Dr. Andreas Bubert explains the Alicyclobacillus spp. test procedure according to the new IFU Method No. 12:2019.
As a worldwide leading provider of a broad range of granulated and ready-to-use culture media for food, beverage and water microbiology, we closely track and support the development of relevant standards aimed at increasing consumer confidence and safety.
Our GranuCult® granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ and ReadyTube® ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.
Figure 1.GranuCult® Prime BAT Agar and BAT Broth
Figure 2.Alicyclobacillus spp. on BAT Agar