D2944

Anti-D₁ Dopamine Receptor antibody,Rat monoclonal

clone 1-1-F11 s.E6, purified from hybridoma cell culture

• Synonym(s): Anti-DRD1
• MDL number: MFCD00282304
• UNSPSC Code: 12352203
• NACRES: NA.25

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1-1-F11 s.E6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: rat, monkey, human
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: 5-10 μg/mL using D₁ dopamine receptor transfected HEK-293T cells; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2a
• UniProt accession no.: P21728
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DRD1(1812) ; rat ... Drd1a(24316)

Description

General description

D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases.

Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors.

Immunogen

recombinant fusion protein containing the C-terminal 97 amino acid of human D₁ dopamine receptor.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in:

• immunohistochemistry
• immunoblotting
• immunocytochemistry
• proximity ligation assay
• co-immunoprecipitation
• western blotting

Biochem/physiol Actions

D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

Sold under exclusive license from Emory University

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves