Typically 3 years from sterilization.
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About This Item
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material
polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Steritop®
feature
hydrophilic
packaging
pack of 12 ea
manufacturer/tradename
Steritop®
parameter
45 °C max. temp.
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
148 mm
diam.
12.5 cm
disc diam.
73 mm
filter capacity
500 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet funnel
45 mm outlet thread(s)
shipped in
ambient
1 of 4
This Item | S2GPU05RE | S2GPT01RE | S2GVT05RE |
|---|---|---|---|
| manufacturer/tradename Steritop® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| capacity 500 mL | capacity 500 mL | capacity 150 mL | capacity 500 mL |
| material polyethersulfone membrane, polystyrene housing, polystyrene funnel(s) | material polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material PVDF membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing |
| disc diam. 73 mm | disc diam. - | disc diam. - | disc diam. - |
| filter diam. 73 mm | filter diam. 73 mm | filter diam. 73 mm | filter diam. 73 mm |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
General description
Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop® 500 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
Legal Information
related product
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
Select the right sterile filter by media, volume, and application.
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Related Content
What are you filtering out? What do you want to keep? Pick the right membrane for your filtration.
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
This article shows that a PES membrane containing Stericup Quick Release filter provides an alternative for the pluripotent expansion of embryonic stem cells.
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What is the expiration date of Stericup® and Steritop® filters?
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Can Stericup® and Steritop® filters be reused?
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All Stericup® and Steritop® filters were developed for single use, disposable processing.
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
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Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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What leachables are detected during filtration processes using Stericup® filters?
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We have not performed extractables/leachables studies for our Stericup® filters.
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
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You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
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No, all Stericup® and Steritop® devices are Research Use Only.
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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What is the maximum vacuum pressure for filtration?
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700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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The User Guide says “Use only glass or plastic bottles designed for vacuum applications”. Can you specify which bottles are compatible?
1 answer-
Steritop® Quick Release bottle top units require a vacuum-rated receiver bottle with either a GL33 or GL45 mm closure thread as specified. Steritop® E bottle top units require a vacuum-rated receiver bottle with a GL45 mm closure thread. Bottles no larger than 2 L should be used.
Glass bottles, GL33:
• Wheaton media bottles (glass), 500 mL (Product Z364886)
• Wheaton media bottles (glass), 1 L (Product Z364894)
Glass bottles, GL45
• Corning® glass media storage bottle, 125 mL (Product CLS1399125)
• Corning® glass media storage bottle, 250 mL (Product CLS1399250)
• Corning® glass media storage bottle, 500 mL (Product CLS1399500)
• Corning® glass media storage bottle, 1 L (Product CLS13991L)
• Pyrex® round media storage bottle, 500 mL (Product CLS1395500)
• Pyrex® round media storage bottle, 1 L (Product CLS13951L)
• KIMBLE® KIMAX® Media bottle, 250 mL (Product DWK14395250)
• KIMBLE® KIMAX® Media bottle, 500 mL (Product DWK14395500)
• KIMBLE® KIMAX® Media bottle, 1 L (Product DWK143951000)
Plastic (pre-sterile) bottles, GL45:
• Stericup® Quick Release Receiver flask, 250 mL (Product S200B02RE)
• Stericup® Quick Release Receiver flask, 500 mL (Product S200B05RE)
• Stericup® Quick Release Receiver flask, 1000 mL (Product S200B10RE)Helpful?
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