700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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About This Item
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material
polyethersulfone membrane
polystyrene funnel(s)
polystyrene housing
Quality Level
sterility
sterile; irradiated
product line
Steritop®
feature
hydrophilic
packaging
pack of 12 ea
parameter
45 °C max. temp.
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
169 mm
diam.
13.5 cm
filter capacity
1000 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet funnel
45 mm outlet thread(s)
shipped in
ambient
1 of 4
This Item | S2GPT02RE | S2GPT01RE | SCGPS01RE |
|---|---|---|---|
| sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| material polyethersulfone membrane, polystyrene housing, polystyrene funnel(s) | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
| fitting inlet funnel | fitting inlet funnel, 45 mm outlet thread(s) | fitting inlet funnel, 45 mm outlet thread(s) | fitting inlet funnel, 33 mm outlet thread(s) |
General description
Stericup® and Steritop® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop® 1000 mL bottle-top with 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 45 mm openings, designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
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Certificates of Analysis (COA)
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Articles
Select the right sterile filter by media, volume, and application.
Evaluation of sterile filtration devices for protein preservation in sterile filtration of cell culture media.
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What is the maximum vacuum pressure for filtration?
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
1 answer-
You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
1 answer-
All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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Can Stericup® and Steritop® filters be reused?
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All Stericup® and Steritop® filters were developed for single use, disposable processing.
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
1 answer-
Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What leachables are detected during filtration processes using Stericup® filters?
1 answer-
We have not performed extractables/leachables studies for our Stericup® filters.
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Are Stericup® and Steritop® filters GMP-certified?
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No, Stericup® and Steritop® devices are not GMP-approved.
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