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Merck

230251

Eosin Y

75% (HPLC)

Synonym(s):

2′,4′,5′,7′-Tetrabromofluorescein, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, Eosin yellowish, Solvent red 43

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About This Item

Empirical Formula (Hill Notation):
C20H8Br4O5
CAS Number:
15086-94-9
Molecular Weight:
647.89
Colour Index Number:
45380:2
EC Number:
239-138-3
MDL number:
MFCD00036189
UNSPSC Code:
12171500
PubChem Substance ID:
24853789
NACRES:
NA.47

Key Documents

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Quality Level

100

Assay

75% (HPLC)

form

powder

color

orange to very dark orange

solubility

spirit: soluble

λmax

524 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5ccccc45)c1Br

InChI

1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI key

DBZJJPROPLPMSN-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
E4009861006212954
Eosin Y 75% (HPLC)

Sigma-Aldrich

230251

Eosin Y

Eosin Y Dye content ~99 %

Sigma-Aldrich

E4009

Eosin Y

Eosin B certified by the BSC, Dye content 90 %

Sigma-Aldrich

861006

Eosin B

Eosin B Dye content 95 %

Sigma-Aldrich

212954

Eosin B

color

orange to very dark orange

color

light red to red/pink

color

-

color

-

solubility

spirit: soluble

solubility

ethanol: 1 mg/mL

solubility

H2O: 1 mg/mL, clear, red

solubility

spirit: soluble

form

powder

form

powder

form

powder

form

powder, crystals or chunks

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

assay

75% (HPLC)

assay

-

assay

-

assay

-

General description

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.[1][2]

Application

Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualize alginate.[3][4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000453030
0000453030
SHBS5803
MKCK7973
MKCD3439

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Gregorios J B
Histophatologic Techniques (2006)
Concordance among pathologists in the second Cardiac Allograft Rejection Gene Expression Observational Study (CARGO II).
Crespo-Leiro MG, Zuckermann A, Bara C, et al.
Transplantation, 94(11), 1172-1177 (2012)
Morphometric dimensions of the spermatozoa in Thai native boar depend onthe ejaculates
Nasinprom N
Animal Reproduction, 540-543 (2015)
Electrophoretic detection of alginate by Hematoxylin and Eosin fluorescence: Implications in cell encapsulation/tissue engineering
Verma Y K
Reactive functional Polymers, 102, 130-136 (2016)
John D. Bancroft
Theory and Practice of Histological Techniques (2008)
View All Related Papers

Protocols

Determination of Silver in Water and Wastewater: Photometric Determination Using Phenanthroline Method and Eosin Y

The article describes the sample preparation and photometric determination of silver concentration in water samples using Spectroquant® photometers and test kits.

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