Enzyme-Linked Immunosorbent Assay (ELISA) Kits
An ELISA (Enzyme-Linked Immunosorbent Assay) is a multi-well plate-based immunoassay within which one of the assay components, typically an antibody or sample, is adsorbed onto a solid surface, in this case, a plate. Offering rapid, quantitative, and sensitive analyte detection at relatively low cost, ELISAs represent one of the simplest assay formats to perform. Furthermore, the ease of adapting an ELISA to a higher throughput screening method empowers researchers to test large sample numbers in a single run.
Learn more about our highly reproducible Conferma® ELISAs or subscribe to our newsletter.
Products
Rat/Mouse Insulin ELISA Kit
measures and quantifies insulin levels in 10 μL serum or plasma
Multi Species GLP-1 ELISA Kit
measures total GLP-1 levels in 20 µl - 50 µl serum, plasma or cell culture supernatent
Multi Species GLP-1 ELISA Kit
measures and quantifies active GLP-1 (7-36 amide and 7-37amide) in 100 μL plasma, serum or cell culture supernatent
Rat/Human GLP-2 ELISA Kit
detects GLP-2 in 50 μL serum, plasma or cell culture supernatent
Human PYY ELISA Kit
measures and quantifies total PYY levels in 20 μL serum or plasma
Human GIP ELISA Kit
measures and quantify total GIP levels in 20 μL serum, plasma or cell culture samples
Human IL-6 ELISA Kit
detect IL-6 in 50 μL serum, plasma or cell culture supernatent
Human Insulin ELISA Kit
measures and quantify insulin levels in 20 μL serum or plasma
Multi Species GLP-1 ELISA Kit
measures active GLP-1 levels in 50 μL plasma
Human TNF-α ELISA Kit
detects TNF-α in 50 μL serum, plasma or cell culture supernatent
Rat/Mouse Growth Hormone ELISA Kit
measures and quantifies GH levels in 10 μL serum, plasma or cell culture supernatent
Human Leptin ELISA Kit
measures and quantify Leptin levels in 25 μL serum or plasma
Human S100B ELISA Kit
quantifies S100B in 50 μL cerebrospinal fluid, serum or plasma
Human Ghrelin ELISA Kit
measures and quantify active Ghrelin levels in 20 μL serum or plasma
Rat/Mouse Ghrelin ELISA Kit
measures and quantifies active Ghrelin in 20 μL serum or plasma
Rat/Mouse FGF-21 ELISA Kit
measures and quantifies FGF-21 levels in 10 µl - 20 µl serum, plasma or cell culture supernatent
Rat/Mouse GIP ELISA Kit
measures and quantifies GIP levels in 10 μL serum, plasma or cell culture supernatent
Mouse Leptin ELISA Kit
measures and quantifies Leptin levels in 10 μL serum or plasma
Rat Leptin ELISA Kit
measures and quantifies Leptin levels in 10 μL plasma or serum
Human Amyloid β40 ELISA Kit
measures and quantifies Amyloid β40 levels in 50 μL CSF, cell culture supernatent or plasma
Sandwich, Direct, and Competitive ELISAs
Our ELISAs are a convenient and user-friendly method for studying soluble protein biomarkers in a variety of matrices including serum, plasma, cell culture supernatant, or lysate. Our ELISA offering consists of >1,000 Research Use Only assays, against a wide variety of species including human, mouse, rat, and various other animal models (Agricultural and Companion). Each assay comes with a specific protocol and the required reagents to run a 96-well plate. ELISAs primarily exist in a 96-well format with the vendor providing the reagents necessary to detect and quantitate the target. There are a variety of ELISA methods including Sandwich, Direct, and Competitive.
The sandwich assay uses a pair of monoclonal antibodies (mAbs) against different epitopes against the same target. The primary mAb, bound to the plate, pulls the protein out of the solution while the second mAb is used to complete the “sandwich” and provide the signal which will indicate the presence of the target. A known amount of the recombinant version of the protein is provided with the kit, allowing the user to create a standard curve against which the signal from one of the samples will be interpreted. The majority of our portfolio is in this sandwich ELISA format.
The direct ELISA and competitive assays are rarer. The direct assay uses a single mAb to detect the sample which is bound to the plate. The mAb is then bound by a reporter secondary antibody to provide the signal.
The competitive assay has a known amount of the biomarker pre-bound to the plate. A labeled antibody is co-incubated with the sample which “mops up” the antibody depending on the concentration of the target in the sample. The free antibody is then able to bind to the antigen on the plate and provide a signal after the sample is washed away. In this case, the signal is inversely proportional to the concentration of the target biomarker.
Conferma® ELISAs and Belysa® Immunoassay Curve Fitting Software
The Conferma® ELISA brand consists of sandwich assays designed to minimize lot-to-lot variability at the critical reagent level, a key concern of assay users. Evaluating the individual lots of critical reagents with a range of analytical techniques aligned with a detailed quality control (QC) protocol allows us to control and limit variability over time.
In order to allow scientists to evaluate the reproducibility of their method we further designed the Belysa® Immunoassay Curve Fitting Software. With this tool, a user can examine their sandwich immunoassay to ensure parameters such as %CV (coefficient of variation) and %Recovery were as expected. Then they can move on to compare the standard curves of multiple plates to confirm that their method was consistent.
Related Resources
- Article: ELISA Protocols
This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.
- Article: ELISA Troubleshooting Guide
Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development.
- Brochure: ELISA Kits
There are several ELISA configurations, with the most common formats being sandwich, competitive and signaling assays.
- Article: Conferma® ELISAs
Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.
- Article: Belysa® Software
Explore how to effortlessly monitor immunoassay method reproducibility using Belysa® analysis software.
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