Find more protocols and selection guides in the Molecular Biology Guide.
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relative ease and they have a well-defined genetic system. Most significantly, yeasts have a highly versatile DNA transformation system that can be utilized effectively for protein production.
For general experimental purposes, yeasts are usually grown in YPD or synthetic media at 30 °C. The following growth media and supplements are available from us for yeast cultures:
For further details regarding yeast media, please refer to our Article, Introduction to Yeast Media.
The protocols listed below can be used for growing and maintaining yeasts in liquid and solid media
Note: Single yeast colonies may be observed after around 24 hours, but incubations over 48 hours are needed before they can be used for replica plating purposes. The growth rate of yeast cultures using synthetic drop-out medium supplements is ~50% slower.
Yeast cells grown using the above methods can be stored frozen in glycerol at -70 °C for more than 3 years or in rich medium slants at 4 °C for 6 months to a year. YPD plates with yeast cells can also be stored at 4 °C for 2-3 months sealed with Parafilm®. The protocols for the preservation and revival of yeast cells are listed below: