TRYPSEQM-RO

Roche

胰蛋白酶测序级,改良

from bovine pancreas

质量水平

100

形式

lyophilized (salt-free)

包装

pkg of 4 × 100 μg (11418033001)
pkg of 4 × 25 μg (11418025001)

manufacturer/tradename

Roche

最佳pH

8.0

运输

wet ice

储存温度

2-8°C

一般描述

从牛胰腺中分离得到的胰蛋白酶测序级(经过修饰)是一种高度纯化的特异性蛋白酶,随后进行修饰。

抑制剂:
TLCK、DFP、PMSF、亮肽素、大豆胰蛋白酶抑制剂、鸡蛋来源的胰蛋白酶抑制剂、抑肽酶、α2-巨球蛋白,α1-抗胰蛋白酶、APMSF 和止痛剂。

特异性

胰蛋白酶是一种丝氨酸内肽酶。在 pH 7.5-9 时,特异性水解赖氨酸和精氨酸的蛋白和肽键 C 端。Arg 和 Lys 的酰胺和酯键也发生断裂。以胰岛素(胰岛素 B ox )的氧化 B 链作为底物,验证了胰蛋白酶测序级(修饰)的特异性。将高浓度的胰蛋白酶(测序级)(经修饰,一份按重量酶加 9 份按重量胰岛素 B ox )孵育 18 小时,以检测痕量糜蛋白酶杂质。

应用

使用经修饰的胰蛋白酶测序级,从纯化的糖蛋白中生成糖肽。
用于:
  • 蛋白质结构解析
  • 胰蛋白酶消化图谱
  • 指纹分析
  • 序列分析
  • 易位研究
  • 蛋白鉴定

质量

纯度:不含可能干扰反相 HPLC 肽分离的杂质。

制备说明

工作浓度: 按重量 1/100~1/5 蛋白
保存条件(工作液):-15~-25℃
胰蛋白酶测序级,经过改造,即使在 pH 值处于中性和弱碱性范围时,也较耐自溶。该酶可以高浓度使用。
2-8°C 条件下储存时,1% 乙酸或 1 mM hcl 溶液的稳定期限为 1 周。-15--25°C 条件下储存时,该溶液至少可稳定 1 年而不损失活性。

其他说明

仅用于生命科学研究。不可用于诊断。

象形图

Exclamation markHealth hazard

警示用语:

Danger

hazcat

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Scott J Walmsley et al.
Journal of proteome research, 12(12), 5666-5680 (2013-10-15)
Trypsin is an endoprotease commonly used for sample preparation in proteomics experiments. Importantly, protein digestion is dependent on multiple factors, including the trypsin origin and digestion conditions. In-depth characterization of trypsin activity could lead to improved reliability of peptide detection...
Desalegn Begna et al.
Journal of proteome research, 10(9), 4263-4280 (2011-07-15)
Despite their similar genetic makeup, honeybee (A. mellifera) queens and workers show alternative morphologies driven by nutritional difference during the larval stage. Although much research have been done to investigate the causes of honeybee caste polymorphism, information at subcellular protein...
Jianke Li et al.
PloS one, 5(10), e13455-e13455 (2010-10-27)
Honeybee (Apis mellifera) exhibits divisions in both morphology and reproduction. The queen is larger in size and fully developed sexually, while the worker bees are smaller in size and nearly infertile. To better understand the specific time and underlying molecular...
Xuchu Wang et al.
Molecular & cellular proteomics : MCP, 12(8), 2174-2195 (2013-05-11)
Thellungiella halophila, a close relative of Arabidopsis, is a model halophyte used to study plant salt tolerance. The proteomic/physiological/transcriptomic analyses of Thellungiella plant leaves subjected to different salinity levels, reported herein, indicate an extraordinary ability of Thellungiella to adapt to...

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