1 base (K3PO4, pre-weighed solid)
4 solvents (DMAc, Toluene, DMSO, and Dioxane)
6 pre-weighed ligands
1 Cu catalyst (Soluble CuI source)
Additional (not included) items needed:
Quench Solution Recipe
Note: This recipe makes 50 mL which is enough stock solution for all four screening sets in the KitAlysis™ Suzuki-Miyaura Cross-Coupling Reaction Screening Kit. The amount of internal standard is 10 mol%. So a big product peak to small internal standard indicates a good reaction.
Work-up Procedure and Analysis
Sealing the Plate-screwing down the cover
Sealing the plate properly is critical to success. The key is light, even pressure to the lid of the block to keep the cover flat while sealing.
Probe for hot plate does not fit into reaction block hole
Use a small oil bath or other metal block (for best results) in the back of the hot plate and place the probe in there. Place reaction block as close to the center of the hot plate as possible for more even stirring.
Due to narrow opening of pipette tips, slurry additions will result in blockages. To aid in uniform dosing, simply snip off the end of the tip at the first marker (~10 mm). To ensure an evenly dispersed aliquot, it is critical that the mixture is stirring well while you draw aliquots for dosing into the corresponding reaction vial.