Pepsin from porcine gastric mucosa

Name: Pepsin from porcine gastric mucosa
Brand: SIGMA
Price: 215 ILS

powder, slightly beige, ≥500 U/mg

Synonym(s):

Pepsin A, Pepsin from hog stomach

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25 G

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About This Item

CAS Number:

9001-75-6

UNSPSC Code:

12352204

eCl@ss:

42010127

EC Number:

232-629-3

NACRES:

NA.54

MDL number:

MFCD00081840

EC Number:

3.4.23.1 (BRENDA, IUBMB)

Key Documents

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Product Name

Pepsin from porcine gastric mucosa, powder, slightly beige, ≥500 U/mg

form

powder

specific activity

≥500 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

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This Item	77151	P7125	P6887
Sigma-Aldrich 77160 Pepsin from porcine gastric mucosa	Sigma-Aldrich 77151 Pepsin from porcine gastric mucosa	Sigma-Aldrich P7125 Pepsin from porcine gastric mucosa	Sigma-Aldrich P6887 Pepsin from porcine gastric mucosa
Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)
specific activity ≥500 U/mg	specific activity 1200-2400 U/mg	specific activity ≥400 units/mg protein	specific activity ≥3,200 units/mg protein
form powder	form powder	form powder	form lyophilized powder
mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa
UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

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Other Notes

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

Peptide synthesis,[1][2] Review.[3]

Peptide synthesis,citationcitation Review.citation

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Synthesis of tetrapeptide p-nitroanilides catalyzed by pepsin.

C A Abdel Malak et al.

International journal of peptide and protein research, 41(2), 97-101 (1993-02-01)

Swine pepsin at pH 5 efficiently catalyzes a condensation between Z-Ala-Ala-Phe-OH and p-nitroanilides of Leu, Phe, Val, Ala and Arg that leads to formation of corresponding benzyloxycarbonyl-tetrapeptide p-nitroanilides with yields of 70-90%. These reactions are complicated by co-precipitation of pepsin

J.S. Fruton

Carlsberg Research Communications, 49, 41-41 (1984)

An Irgafos® 168 story: When the ubiquity of an additive prevents studying its leaching from plastics.

Ludovic Hermabessiere et al.

The Science of the total environment, 749, 141651-141651 (2020-08-25)

Plastic pollution is a source of chemical to the environment and wildlife. Despite the ubiquity of plastic pollution and thus plastic additive in the environment, plastic additives have been studied to a limited extend. As a prerequisite to a study

M.P. Bemquerer et al.

Biomedica Biochimica Acta, 50 (1991)

Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro.

Abdelhacib Kihal et al.

Animals : an open access journal from MDPI, 11(8) (2021-08-28)

The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of

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